Syllabus (BOTANY)

Course Type: MAJ-13

Semester: 7

Course Code: BBOTMAJ13C

Course Title: Immunology and Applied Microbiology

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: The main objectives of studying applied microbiology are to exploit microorganisms for a specific product or use. Applied microbiology can overlap with basic microbiology, where methods used and perfected for applied microbiology can become tools for basi

Learning Outcome: Students will learn about cells and molecules of Innate and adaptive immune system Students will learn about antibody molecules helps in detection of biomolecules Students will learn about bioreactors/fermenters and fermentation processes Students will le

(Theory, Credit 4)

Unit	Topic	No. of Lectures/Hrs
1	Innate and adaptive immune system, Cells and molecules involved in innate and adaptive immunity, antigens, antigenicity and immunogenicity.	6
2	B and T cell epitopes, structure and function of antibody molecules. generation of antibody diversity, monoclonal antibodies Antibody generation, antigen-antibody interactions, MHC molecules, antigen processing and presentation, activation and differentiation of B and T cells, B and T cell receptors, humoral and cell mediated immune responses, primary and secondary immune modulation, the complement system, Toll-like receptors, cell-mediated effector functions, Chemokine and cytokine , inflammation, hypersensitivity and autoimmunity, congenital and acquired immunodeficiencies, vaccines types.	16
3	Reas: Detection of molecules using ELISA, RIA, western blot, immunoprecipitation, detection of molecules in living cells, in situ localization by techniques such as FISH and GISH.	8
4	Bioreactors/Fermenters and fermentation processes, Solid-state and liquid-state fermentations; Batch fed batch and continuous fermentations, Components of a typical bioreactor, Types of bioreactors-laboratory, Constantly stirred tank fermenter, tower fermenter, fixed bed and fluidized bed bioreactors and air-lift fermenter.	8
5	Microbial production of industrial products in nutrition, healthcare and agriculture, Antimicrobial drugs; Antibiotics: Classification molecular mechanism of mode of action and resistance; Antifungal and antiviral drugs, Amino acids, vitamins, enzymes, organic acids, antitumor, immune suppressants, Biofuel bioplastic, waste water treatment.	8
6	Downstream processing and uses; Filtration, centrifugation, cell disruption, solvent extraction, precipitation and ultrafiltration, lyophilization, spray drying	6
7	Microbial interactions; Carbon, sulphur, phosphorus and nitrogen cycles in forest soil and agriculture soil, Soil microorganisms associated with vascular plants (endophytes, epiphytes), Biological Nitrification Inhibition (BNI), Bioremediation, Uncultivable microorganisms, Process of making microbial inoculants, Top down, Bottom up, Mixed, Challenge with the establishment of microbial inoculants.	8

Practical (Credits -2, 60 Hrs)

Hands on microbial fermentations for the production and estimation (qualitative and quantitative) of Enzyme: amylase urease, phosphatase, lipase activity

Reading References on Immunology:

1. Cellular and Molecular Immunology- Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai

2. Roitt's Essential Immunology- Peter J. Delves, Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt

3. Kuby Immunology- Richard A. Goldsby, Thomas J. Kindt, Barbara A. Osborne, Janis

4. Immunology –Saras Publication

Reading References of Applied Microbiology:

1. Prescott L.M; J.P Heavy and D.A. Klein 1993 Microbiology 7th edition Wm C- Brown Publishers

2. Michael T. Madigan, John M. Martinko, Kelly Bender, Daniel P Buckley, David A. Stahl. Brock Biology of Microorganisms, 13th edition, Prentice hall International, Inc

3. Atlas, R.M. 1997. Principles of Microbiology, Second edition. WCK. McGraw – Hill. Prescott LM, Harley JP and Klein DA (2007) Microbiology (7th Edition) McGraw Hill New York

4. Ananthanarayanan, R. and Jayaram Panicker C.K. (2013) Text book of Microbiology. 9th Edition University Press, Hyderabad.

5. Stanbury, P.F., A. Whitaker and Hall S.J. 1995. Principles of fermentation technology. 2nd edition., Pergamon press. Casida, J.E. 1968: Industrial Microbiology, Wiley Eastern. Pastel, A.H. 2012; Industrial Microbiology. 2nd edition. Macmillan India Limited. Cruger and Cruger, Textbook of Industrial Microbiology. 2004

6. Principles of Fermentation Technology by P.F. Stanbury, W. Whitaker &S.J. Hall, Aditya Books (P) Ltd., New Delhi, 1997.

7. Peppler, H. J. and Pearlman, D. 1979. Microbial Technology, Vol 1 ans 2, Academic press.

8. Industrial Microbiology: An Introduction by Waites, Morgan, Rockey & Highton, Blackwell Science, 2001.

9. Biochemical Engineering and Biotechnology by B. Atkinson & F. Mavituna, The Nature Press, 1982. Bioreactor Design and Product Yield (1992), BIOTOL series, Butterworths

10. Chand, Fermentation Biotechnology: Industrial Perspectives.