Sidho-Kanho-Birsha University
Syllabus & Course Curriculam
Syllabus & Course Curriculam
Course Type: MAJ-7
Semester: 5
Course Code: BBOTMAJ07C
Course Title: Plant Physiology and Metabolism
(L-P-Tu): 4-2-0
Credit: 6
Practical/Theory: Combined
Course Objective: Understand the role of physiological and metabolic processes for plant growth and development. Recognizing the wonderful mechanism of transport and the Interrelationships existing between metabolic pathways thereby gaining and idea about the importance of
Learning Outcome: After the completion of the course the students will be able to: 1. Understand basic functions and intermediary metabolism in a plant body. 2. Gain knowledge on the role of physiological and metabolic processes for plant growth and development. 2. Lear
(Theory, Credit 4)
| Unit | Topic | No. of Lectures/Hrs |
| 1 | Plant-water relations: Water Potential and its components, water absorption by roots, aquaporins, pathway of water movement, symplast, apoplast, transmembrane pathways, root pressure, guttation. Ascent of sap– cohesion-tension theory. Transpiration and factors affecting transpiration, antitranspirants, mechanism of stomatal movement. | 5 |
| 2 | Mineral nutrition: Essential and beneficial elements, macro and micronutrients, methods of study and use of nutrient solutions, criteria for essentiality, mineral deficiency symptoms, roles of essential elements, chelating agents. Transport of ions across cell membrane. | 5 |
| 3 | Translocation in the phloem: Pressure–Flow Model; Phloem loading and unloading; Source–sink relationship. | 4 |
| 4 | Plant growth regulators: Discovery, chemical nature (basic structure), bioassay and physiological roles of Auxin, Gibberellins, Cytokinin, Abscisic acid and Ethylene, | 5 |
| 6 | Plant response to light and temperature: Photoperiodism (SDP, LDP, Day neutral plants); Phytochrome (discovery and structure), red and far-red light responses, Vernalization. | 4 |
| 7 | Concept of metabolism: Introduction, anabolic and catabolic pathways, regulation of metabolism, role of regulatory enzymes (allosteric, and Isozymes). | 3 |
| 8 | Photosynthesis: photosynthetic pigments (chlorophylls and accessory pigments), antenna molecules and reaction centres, photosynthetic electron transport, PSI, PSII, Q cycle, CO2 reduction, photorespiration, C4 pathways; CAM; Factors affecting CO2 reduction. | 9 |
| 9 | Respiration: Glycolysis, regulation of glycolysis, oxidative pentose phosphate pathway, oxidative decarboxylation of pyruvate, regulation of PDH, NADH shuttle; TCA cycle, amphibolic role, anaplerotic reactions, regulation of the cycle, cyanide-resistant respiration, factors affecting respiration. | 8 |
| 10 | ATP-Synthesis: Mechanism of ATP synthesis, substrate level phosphorylation, chemiosmotic mechanism (oxidative and photophosphorylation), ATP synthase, role of uncouplers. | 4` |
| 11 | Lipid metabolism: Synthesis and breakdown of triglycerides, β-oxidation, glyoxylate cycle, gluconeogenesis and its role in mobilisation of lipids during seed germination. | 4 |
| 12 | Nitrogen metabolism: Biological nitrogen fixation; Nitrate and ammonia assimilation. | 4 |
| 13 | Signal transduction: Hormones and their receptors, cell surface receptor, signaling through G-protein coupled receptors, signal transduction pathways, second messengers, Calcium calmodulin, MAP kinase cascade. | 5 |
Practical (Credits -2, 60 Hrs)
1. Determination of osmotic potential of plant cell sap by plasmolytic method.
2. Determination of water potential of given tissue (potato tuber) by weight method.
3. Study of the effect of wind velocity and light on the rate of transpiration in excised twig/leaf.
4. Calculation of stomatal index and stomatal frequency from the two surfaces of leaves of a mesophyte and xerophyte.
5. To study the phenomenon of seed germination (effect of light).
6. Experimental demonstration of Hill’s reaction.
7. To study the effect of light intensity and bicarbonate concentration on O2 evolution in photosynthesis.
8. To compare the rate of respiration in different parts of a plant.
Demonstration experiments
1. To demonstrate suction due to transpiration.
2. Fruit ripening/Rooting from cuttings (Demonstration).
3. Bolting experiment/Avena coleptile bioassay (demonstration).
4. Effect of auxins on rooting.
5. R.Q.
Reading References:
1. Hopkins, W.G. and Huner, A. (2008). Introduction to Plant Physiology. John Wiley and Sons. U.S.A. 4th edition.
2. Taiz, L., Zeiger, E., MØller, I.M. and Murphy, A (2015). Plant Physiology and Development. Sinauer Associates Inc. USA. 6th edition.
3. Bajracharya D. (1999). Experiments in Plant Physiology-A Laboratory Manual. Narosa Publishing House, New Delhi.
4. Harborne, J.B. (1973). Phytochemical Methods. John Wiley & Sons. New York.
Basic Features
Undergraduate degree programmes of either 3 or 4-year duration, with multiple entry and exit points and re-entry options, with appropriate certifications such as:
Note: The eligibility condition of doing the UG degree (Honours with Research) is- minimum75% marks to be obtained in the first six semesters.
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