Syllabus (MICROBIOLOGY)

Course Type: ME-2

Semester: 2

Course Code: BMCBMEB12C

Course Title: INTRODUCTION TO MICROBIOLOGY & MICROBIAL DIVERSITY

(L-P-Tu): 2-2-0

Credit: 4

Practical/Theory: Combined

Course Objective: 1.    To learn the basic idea about world of microorganisms, historical developments and major milestones in the field of microbiology; microscopy and staining techniques for study of microorganisms. 2.    To study the diversity of microbial world.

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1.    Understand the history of microbiology and identify and classify diverse group of prokaryotic and eukaryotic organisms 2.    Acquaint oneself with microscopy, staining techniques and bacteriological techniques used in microbiology. 3.    Know about physical and chemical method of sterilization.

MINOR COURSE-II ( TO BE SELECTED FROM THE LIST OF MINOR POOL-2)

COURSE TITLE: INTRODUCTION TO MICROBIOLOGY AND MICROBIAL DIVERSITY (THEORY)

TOTAL HOURS: 45 CREDITS:3

Unit 1. History of Development of Microbiology No. of hours: 10

Development of microbiology as a discipline, Spontaneous generation vs. biogenesis. Contributions of Anton von Leeuwenhoek, Louis Pasteur, Robert Koch, Joseph Lister, Alexander Fleming. Germ theory of disease, Development of various microbiological techniques and golden era of microbiology.

Unit 2. Diversity of Microorganisms No. of Hours: 15

Systems of classification: Binomial nomenclature, Whittaker’s five kingdom and Carl Woese’s three kingdom classification systems and their utility General characteristics of different groups: Acellular microorganisms (Viruses) and Cellular microorganisms (Prokarya: Archaea and Bacteria, Eukarya: Algae, Fungi and Protozoa) giving definitions and citing examples Protozoa: Methods of nutrition, locomotion & reproduction - Amoeba, Paramecium

Unit 3. Microscopy and staining techniques No. of hours: 12

Bright Field Microscope, Dark Field Microscope, Phase Contrast Microscope, Fluorescence Microscope, Electron Microscope- SEM & TEM. Classification of stains; Principles of staining; Negative, Simple & Differential staining.

Unit 4. Control of microorganism No. of hours: 8

Physical methods of microbial control: heat, low temperature, high pressure, filtration, desiccation, osmotic pressure, radiation.

Chemical methods of microbial control: disinfectants, types and mode of action

SUGGESTED READINGS

1. Tortora GJ, Funke BR and Case CL. (2008). Microbiology: An Introduction. 9th edition.Pearson Education 2. Madigan MT, Martinko JM, Dunlap PV and Clark DP. (2014). Brock Biology of Microorganisms. 14th edition. Pearson International Edition

3. Cappucino J and Sherman N. (2010). Microbiology: A Laboratory Manual. 9th edition. Pearson Education Limited

4.Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology.9th Edition. McGraw Hill International.

5. Atlas RM. (1997).Principles of Microbiology.2nd edition.WM.T.Brown Publishers.

6. Pelczar MJ, Chan ECS and Krieg NR.(1993). Microbiology.5th edition. McGraw Hill Book Company.

7. Stanier RY, Ingraham JL, Wheelis ML, and Painter PR. (2005).General Microbiology.5th edition. McMillan.

COURSE TITLE: INTRODUCTION TO MICROBIOLOGY AND MICROBIAL DIVERSITY (PRACTICALS)

TOTAL HOURS: 30 CREDITS:1

Microbiology Good Laboratory Practices and Biosafety
To study the principle and applications of important instruments (laminar air flow, autoclave, incubator, hot air oven, light microscope, pH meter) used in the microbiology laboratory.
Staining Techniques: Simple Staining, Negative Staining, Gram Staining
Study of Spirogyra and Volvox using permanent Mounts.
Study of Paramecium and Euglena using permanent Mounts.
Study of Penicillium, Aspergillus using permanent mounts