Syllabus (MICROBIOLOGY)

Course Type: MAJ-9

Semester: 6

Course Code: BMCBMAJ06T

Course Title: CELL BIOLOGY & MICROBIAL GENETICS

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: 1. To learnthe scope and importance of recombinant DNA technology. 2. To study the different molecular methods and vectors used widely used in RDT. 1. To study the types and functioning of different organelles and mechanisms in eukaryotic and prokaryotic cells. 2. To provide detailed knowledge on fine structure and function of the cell. 3. To study mechanisms of gene transfer, expression and regulation. 4. To comprehend the types and effects of mutations and recombination.

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1. Gain knowledge of functioning of different part of cells and understand differences between normal and diseased cells. 2. Understand the organization of cell mechanisms of cell-to-cell communication. 3. Gain knowledge of prokaryotic gene transfer mechanisms. 4. Understand mutations and recombination.

MAJOR COURSE 02

COURSE TITLE: CELL BIOLOGY AND MICROBIAL GENETICS (THEORY)

Total hours: 60 Credits: 4

Unit 1. Structure and organization of Cell No. of hours: 12

Cell Organization–Eukaryotic (Plant and animal cells) and prokaryotic Plasma membrane: Structure and transport of small molecules, Cell Wall: Eukaryotic cell wall, Extracellular matrix and cell matrix interactions, Cell-Cell Interactions and plasmodesmata (only structural aspects) Mitochondria, chloroplasts peroxisomes and Cytoskeleton.

Unit 2. Cell Signalling No. of hours: 8

Signalling molecules and their receptors, Function of cell surface receptors, Pathways of intra-cellular receptors–Cyclic AMP pathway, cyclic GMP and MAP kinase pathway.

Unit 3. Mutations No. of hours: 9

Mutations and mutagenesis: Definition and types of Mutations. Physical and chemical Mutagens.Uses of mutations, DNA damage and repair mechanisms.

Unit 4.Plasmids and Transposable Elements No. of hours: 12

Types of plasmids – F plasmid, R Plasmids, colicinogenic plasmids, Ti plasmids, linear plasmids, yeast- 2 μ plasmid, Host range, plasmid-incompatibility, plasmid amplification, Regulation of copy number, curing of plasmids. Prokaryotic transposable elements – Insertion Sequences, composite and non-composite transposons, Replicative and Non replicative transposition, Uses of transposons and transposition.

Unit 5. Mechanisms of Genetic Exchange No. of hours: 12

Transformation, Conjugation, Hfr and F’strains, Interrupted mating technique Transduction-Generalized transduction, specialized transduction, LFT & HFT lysates.

SUGGESTED READING

1. Klug WS, Cummings MR, Spencer, C, Palladino, M (2011). Concepts of Genetics, 10^th Ed., Benjamin Cummings.

2. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and Bartlett Learning.

3. Pierce BA (2011) Genetics: A Conceptual Approach, 4th Ed., Macmillan Higher Education Learning.

4. Watson JD, Baker TA, Bell SP et al. (2008) Molecular Biology of the Gene, 6th Ed., Benjamin Cummings.

5. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India.

6. Russell PJ. (2009). i Genetics- A Molecular Approach. 3rd Ed, Benjamin Cummings.

7. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition, Cold Spring Harbour Laboratory press.

8. Maloy SR, Cronan JE and Friefelder D(2004) Microbial Genetics 2nd EDITION., Jones and Barlett Publishers.

COURSE TITLE: Cell biology and Microbial Genetics (PRACTICAL)

Total hours: 60 Credits: 2

1. Preparation of Master and Replica Plates

2. Study the effect of chemical (HNO₂) and physical (UV) mutagens on bacterial cells

3. Study survival curve of bacteria after exposure to ultraviolet (UV) light

4. Demonstration of Bacterial Conjugation

5. Demonstration of bacterial transformation and transduction

6. Demonstration of AMES test