Syllabus (MICROBIOLOGY)

Course Type: MAJ-6

Semester: 5

Course Code: BMCBMAJ05T

Course Title: MOLECULAR BIOLOGY

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: 1. To study the process of replication, transcription and translation in both prokaryotes and eukaryotes. 2. To study the mechanism of gene regulation in prokaryotes.

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1. Acquaint oneself with the knowledge on DNA replication in prokaryotes. 2. Knowthe mechanism of process of transcription and translation in prokaryotes. 3. Learn the comparative stu

MAJOR COURSE 01

COURSE TITLE: MOLECULAR BIOLOGY (THEORY)

TOTAL HOURS: 60 CREDITS:4

Unit 1. Replication of DNA in ProkaryotesNo. of hours: 15

Bidirectional and unidirectional replication, semi- conservative, semi- discontinuous replication Mechanism of DNA replication: Enzymes and proteins involved in DNA replication –DNA polymerases, DNA ligase, primase, telomerase – for replication of linear ends, Various models of DNA replication including rolling circle, D- loop (mitochondrial), Ө (theta) mode of replication and other accessory protein, Comparative study of DNA replication of Prokaryotes and eukaryotes.

Unit 2. Transcription in Prokaryotes No. of hours: 12

Transcription: Definition, difference from replication, promoter - concept and strength of promoter, RNA Polymerase, Comparative study of transcription of prokaryotes and eukaryotes, Inhibitors of RNA synthesis.

Unit 3. Translation in Prokaryotes No. of hours: 15

Genetic code and its characteristics, Charging of tRNA, aminoacyltRNAsynthetases, Mechanisms of initiation, elongation and termination of polypeptides in prokaryotes, comparative study of translation of prokaryotes with that of eukaryotes, Inhibitors of protein synthesis in prokaryotes and eukaryote.

Unit 4. Post transcriptional modifications & post translational modifications No. of hours: 8

Post transcriptional modifications -Split genes, concept of introns and exons, RNA splicing, spliceosome machinery, concept of alternative splicing, Polyadenylation and capping, Processing of rRNA, RNA interference: si RNA, miRNA and its significance. Post-translational modifications-chemical modification, proteolytic cleavage, protein splicing

Unit 5. Gene Regulation No. of hours: 10

Positive control and negative control, Inducible system and repressible system, Lac operon, Catabolite repression., Trp operon

SUGGESTED READING

1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of theGene, 6th edition, Cold Spring Harbour Lab. Press, Pearson Publication
2. Becker WM, Kleinsmith LJ, Hardin J and Bertoni GP (2009) The World of the Cell, 7th edition,Pearson Benjamin Cummings Publishing, San Francisco
3. De Robertis EDP and De Robertis EMF (2006) Cell and Molecular Biology, 8th edition. Lippincott Williams and Wilkins, Philadelphia
4. Karp G (2010) Cell and Molecular Biology: Concepts and Experiments, 6th edition, John Wiley &Sons. Inc.
5. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4 th Edition, ColdSpring Harbour Laboratory press.
6. Krebs J, Goldstein E, Kilpatrick S (2013). Lewin’s Essential Genes, 3rd Ed., Jones and BartlettLearning
7. Gardner EJ, Simmons MJ, Snustad DP (2008). Principles of Genetics. 8th Ed. Wiley-India

COURSE TITLE: MOLECULAR BIOLOGY (PRACTICALS)

TOTAL HOURS: 60 CREDITS:2

1. Study of different types of DNA and RNA using micrographs and model/ schematic representations
2. Isolation of plasmid DNA.
3. Isolation of genomic DNA from E. coli.
4. Study of DNA brand by Agarose Gel Electrophoresis.