Syllabus (MICROBIOLOGY)

Course Type: MAJ-5

Semester: 4

Course Code: BMCBMAJ04T

Course Title: INSTRUMENTATION AND BIOTECHNIQUES (THEORY)

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: 1. To learn the working principles, construction and applications of the instruments used in the studies related to various disciplines of biological sciences. 2. To perform biotechniques in the laboratory.

Learning Outcome: Upon successful completion of the course the candidate will be able to - 1. Understand the working principles of different instruments widely used in biological science. 2. Acquaint oneself with different biotechniques used in biological science. 3. Separ

MAJOR COURSE 02

COURSE TITLE: INSTRUMENTATION AND BIOTECHNIQUES (THEORY)

TOTAL HOURS: 60 CREDITS: 4

Unit1. Microscopy No. of hours: 10

Idea about numerical aperture, angular aperture, limit of resolution, refractive index etc. Principle and application of Bright field and dark field microscopy, Fluorescence Microscopy, Phase contrast Microscopy, Electron Microscopy (Scanning and Transmission Electron Microscopy) and Micrometry.

Unit2. Chromatography No. of hours: 20

Principles and applications of paper chromatography (including descending and ascending, radial). Thin layer chromatography. Gel filtration chromatography, ion exchange chromatography and affinity chromatography, GLC, HPLC.

Unit3. Electrophoresis No. of hours: 10

Principle and applications of native polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, 2D gel electrophoresis, isoelectric focusing, Zymogram preparation and Agarose gel electrophoresis,

Unit4. Spectrophotometry No. of hours: 10

Principle and use of study of absorption spectra of biomolecules. Analysis of Biomolecules using UV and visible range. Colorimetry and turbidometry.

Unit 5. Centrifugation No. of hours: 10

Preparative and analytical centrifugation, fixed angle and swinging bucket rotors. RCF And Sedimentation coefficient, differential centrifugation, density gradient Centrifugation and Ultracentrifugation

SUGGESTED READING

1. Wilson & Walker. Principles and Techniques in Practical Biochemistry. 5th Edition. Cambridge University Press (2000).

2. Murphy D.B. Fundamental of Light Microscopy & Electron Imaging. 1 st Edition. Wiley- Liss. (2001).

3. K L Ghatak. Techniques And Methods In Biology. PHI Publication (2011)

4. Pranav Kumar. Fundamentals and Techniques of Biophysics and Molecular Biology (2016).

5. Aurora Blair. Laboratory Techniques & Experiments In Biology. Intelliz Press

6. D.T Plummer. An Introduction to Practical Biochemistry. McGraw Hill Publication 1987

7. Beckner, W.M., Kleinsmith L.J and Hardin J. The world of cell. IV edition. Benjamin Cummings (2000)

8. Biophysical Chemistry by Upadhyay, Upadhyay, Nath. Himalaya Publishing House

COURSE TITLE: INSTRUMENTATION AND BIOTECHNIQUES (PRACTICAL)

TOTAL HOURS: 60 CREDITS: 2

1. Ascending and radial paper chromatography of sugars, amino acids.
2. Thin layer chromatography of sugars and amino acids.
3. SDS-PAGE electrophoresis method.

.