Syllabus (MICROBIOLOGY)

Course Type: MAJ-4

Semester: 4

Course Code: BMCBMAJ04T

Course Title: MICROBIAL PHYSIOLOGY AND METABOLISM (THEORY) MAJOR COURSE 02 COURSE TITLE: INSTRUMENTATION AND BIOTECHNIQUES (THEORY)

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: 1. To learn about microbial growth pattern and effect of environmental factors on microbial growth. 2. Tostudy the microbial metabolism in detailand learn how microbes do catabolism to get energy and metabolism to build structure. 1. To learn the working

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1. To recognize how fundamental chemical principles and reactions are utilized in biochemical processes. 2. To differentiate microbesbased on the metabolism 3. To understand the princ

MAJOR COURSE 01

COURSE TITLE: MICROBIAL PHYSIOLOGY AND METABOLISM (THEORY)

TOTAL HOURS: 60 CREDITS: 4

Unit1: Microbial Growth and Effect of Environment on Microbial Growth No. of hours:20

Definitions of growth, measurement of microbial growth, Batch culture, Continuous culture, generation time and specific growth rate, synchronous growth, diauxic growth curve. Microbial growth in response to environment-Temperature (psychrophiles, Mesophiles, thermophiles, extremophiles, thermoduric, psychographs), PH (acidophiles, Alkaliphiles), solute and water activity (halophiles, xerophilies, osmophilic), Oxygen (aerobic, anaerobic, microaerophilic, facultative aerobe, facultative anaerobe), barophilic. Microbial growth in response to nutrition andenergy– Autotroph/Phototroph, heterotrophy,Chemolithoautotrophic, Chemolithoheterotrophic, Chemoheterotroph, Chemolithotroph, photolithoautotroph, Photo organoheterotroph.

Unit2: Nutrient uptake and Transport No. of hours:12

Passive and facilitated diffusion, Primary and secondary active transport, concept of uniport, symport and antiport, Group translocation.

Unit3: Respiration, concept of aerobic and anaerobic respiration No. of hours:15

Glycolysis, TCA, EMP, ED, PPP, Electron transport chain. Components of mitochondrial and bacterial ETC. Phosphorylation, Uncouplers and inhibitors.

Unit 4. Chemo lithotrophic and Phototrophic Metabolism No. of hours:13

Introduction to aerobic and anaerobic chemolithotroph with an example each. Hydrogen oxidation (definition and reaction) and methanogenesis (definition and reaction), anoxygenic vs. oxygenic photosynthesis with reference to photosynthesis in green bacteria, purple bacteria and cyanobacteria.

SUGGESTED READING

1. Voet&Voet. Fundamentals of biochemistry Wiley
2. M.M. Cox, D. L. Nelson. Lehninger’s principles of biochemistry. W H Freeman
3. Stryer. Biochemistry W H Freeman
4. Fundamentals of Biochemistry (2016) by J L Jain, Sunjay Jain, Nitin Jain. S. Chand
5. Madigan, Martinko, Bender, Buckley, Stahl. Brock Biology of Microorganisms. Pearson
6. Prescott, M.J., Harley, J.P. and Klein, D.A. Microbiology. 5th Edition WCB McGrawHill, New York
7. Madigan MT, and Martinko JM (2014). Brock Biology of Microorganisms.14th edition. Prentice Hall International Inc.
8. Moat AG and Foster JW. (2002). Microbial Physiology.4th edition. John Wiley &Sons
9. Reddy SR and Reddy SM. (2005). Microbial Physiology. Scientific Publishers India
10. Gottschalk G. (1986). Bacterial Metabolism.2nd edition. Springer Verlag
11. Stanier RY, Ingrahm JI, Wheelis ML and Painter PR. (1987). General Microbiology.5th edition, Mc Millan Press.
12. Willey JM, Sherwood LM, and Woolverton CJ. (2013). Prescott’s Microbiology.9^Th edition.

MAJOR COURSE 02

COURSE TITLE: INSTRUMENTATION AND BIOTECHNIQUES (THEORY)

TOTAL HOURS: 60 CREDITS: 4

Unit1. Microscopy No. of hours: 10

Idea about numerical aperture, angular aperture, limit of resolution, refractive index etc. Principle and application of Bright field and dark field microscopy, Fluorescence Microscopy, Phase contrast Microscopy, Electron Microscopy (Scanning and Transmission Electron Microscopy) and Micrometry.

Unit2. Chromatography No. of hours: 20

Principles and applications of paper chromatography (including descending and ascending, radial). Thin layer chromatography. Gel filtration chromatography, ion exchange chromatography and affinity chromatography, GLC, HPLC.

Unit3. Electrophoresis No. of hours: 10

Principle and applications of native polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, 2D gel electrophoresis, isoelectric focusing, Zymogram preparation and Agarose gel electrophoresis,

Unit4. Spectrophotometry No. of hours: 10

Principle and use of study of absorption spectra of biomolecules. Analysis of Biomolecules using UV and visible range. Colorimetry and turbidometry.

Unit 5. Centrifugation No. of hours: 10

Preparative and analytical centrifugation, fixed angle and swinging bucket rotors. RCF And Sedimentation coefficient, differential centrifugation, density gradient Centrifugation and Ultracentrifugation

SUGGESTED READING

1. Wilson & Walker. Principles and Techniques in Practical Biochemistry. 5th Edition. Cambridge University Press (2000).

2. Murphy D.B. Fundamental of Light Microscopy & Electron Imaging. 1 st Edition. Wiley- Liss. (2001).

3. K L Ghatak. Techniques And Methods In Biology. PHI Publication (2011)

4. Pranav Kumar. Fundamentals and Techniques of Biophysics and Molecular Biology (2016).

5. Aurora Blair. Laboratory Techniques & Experiments In Biology. Intelliz Press

6. D.T Plummer. An Introduction to Practical Biochemistry. McGraw Hill Publication 1987

7. Beckner, W.M., Kleinsmith L.J and Hardin J. The world of cell. IV edition. Benjamin Cummings (2000)

8. Biophysical Chemistry by Upadhyay, Upadhyay, Nath. Himalaya Publishing House

COURSE TITLE: MICROBIAL PHYSIOLOGY AND METABOLISM (PRACTICAL)

TOTAL HOURS: 60 CREDITS: 2

1. Study and plot of bacterial growth curve by turbidimetric method.
2. Effect of temperature on growth of E. coli.
3. Effect of pH on growth of E. coli.
4. Effect of Heavy metals on growth of E. coli.
5. Effect of osmotic pressure on growth of E. coli
6. Demonstration of alcoholic fermentation.