Syllabus (MICROBIOLOGY)

Course Type: MAJ-2

Semester: 2

Course Code: BMCBMAJ02T

Course Title: MICROBIAL DIVERSITY

(L-P-Tu): 4-2-0

Credit: 6

Practical/Theory: Combined

Course Objective: 1. To study the diversity of microbial world. 2. To study the structural and economical importance of prokaryotes and eukaryotes 3. To learn about the concept of virus and its replication.

Learning Outcome: Upon successful completion of the course the candidate will be able to – 1. Identify and classify diverse group of prokaryotic and eukaryotic organisms. 2. Understand the structure of viruses, their mode of replication and viral assay.

SEMESTER II

MAJOR COURSE 01

COURSE TITLE: MICROBIAL DIVERSITY (THEORY)

TOTAL HOURS:60 CREDITS:4

Unit 1. General characteristics of prokaryotes No. of hours: 10

General account & economic importance of Mycoplasma, Actinomycetes, Cyanobacteria and Archaea

Unit 2. Fungi No. of hours: 10

General characteristics of fungi including habitat, distribution, structure and reproduction and economic importance

Unit 3. Algae No. of hours:10

General characteristics of algae including habitat, distribution,structure and reproduction and economic importance

Unit 4. Protozoa No. of hours:10

General characteristics of protozoa with special reference to Amoeba, Paramecium, Plasmodium and Giardia

Unit 5. Nature and properties of viruses No. of hours:10

Physiochemical properties of virus, Classification system (ICTV, Baltimore), Chemistry and structural organisation of viruses (TMV, Animal viruses and Bacteriophage). Concept of viroid, virusoids, satellite viruses and prions. Viral assay (plaque assay)

Unit 6. Lytic and lysogenic cycle No. of hours:10

Life cycle of Bacteriophages with special reference to E. coli and T4, Lysogenic conversion, induction and significance, one step multiplication curve.

SUGGESTED READING

1. Atlas RM. (1997)Principles of Microbiology.2nd edition.WM.T.Brown Publishers.
2. Black JG. (2008). Microbiology: Principles and Explorations. 7th edition. Prentice Hall
3. Madigan MT, and MartinkoJM.(2014). Brock Biology of Micro-organisms.14th edition.ParkerJ.Prentice Hall International, Inc.
4. PelczarJr MJ, Chan ECS, and Krieg NR.(2004). Microbiology.5th edition Tata McGraw Hill.
5. Srivastava S and Srivastava PS. (2003). Understanding Bacteria. Kluwer Academic Publishers,Dordrecht
6. Stanier RY, Ingraham JL, Wheelis ML and Painter PR. (2005). General Microbiology. 5th editionMcMillan.
7. Tortora GJ, Funke BR, and Case CL. (2008). Microbiology: An Introduction. 9th edition PearsonEducation.
8. Willey JM, Sherwood LM, and Woolverton CJ.(2013). Prescott’s Microbiology.9th edition.McGraw Hill Higher Education
9. Dimmock, NJ, Easton, AL, Leppard, KN (2007). Introduction to Modern Virology. 6th edition,Blackwell Publishing Ltd.
10. Carter J and Saunders V (2007). Virology: Principles and Applications. John Wiley and Sons.
11. Levy JA, Conrat HF, Owens RA. (2000). Virology.3rd edition. Prentice Hall publication, NewJersey.
12. Wagner EK, Hewlett MJ. (2004). Basic Virology.2nd edition.Blackwell Publishing.
13. Nayudu MV. (2008). Plant Viruses. Tata McGraw Hill, India.
14. Bos L. (1999) Plant viruses-A text book of plant virology by. Backhuys Publishers.
15. Versteeg J. (1985). A Color Atlas of Virology.Wolfe Medical Publication.

COURSE TITLE: MICROBIAL DIVERSITY (PRACTICAL)

TOTAL HOURS:60 CREDITS:2

1. Study of Spirogyra ,Chlamydomonasand Volvox using permanent Mounts.
2. Study of Amoeba, Paramecium and Euglena using permanent Mounts.
3. Study of Penicillium, Aspergillus using permanent mounts
4. Study of Staphylococcus aureus and Lactobacillus using permanent mounts
5. Determination of motility of bacteria by Hanging Drop Technique
6. Determination of viral plaque assay.
7. Isolation of pure cultures of bacteria by streaking method.
8. Estimation of CFU count by spread plate method/pour plate method.