Syllabus (MICROBIOLOGY)

Course Type: SEC-1

Semester: 1

Course Code: BMCBSEC01T

Course Title: AIR AND WATER MICROBIOLOGY

(L-P-Tu): 5-0-1

Credit: 4

Practical/Theory: Theory

Course Objective: To acquaint with the basic concept of aeromicrobiology and water microbiology along with microbiological analysis of air and water.

Learning Outcome: Upon successful completion of the course the candidate will be able to- 1. Understand the concept of bioaerosols, disease causing air-borne microorganisms and its impact on human health. 2. Know aboutthe different kinds of water borne pathogens and water borne diseases. 3. Learn the different techniques used for microbiological analysis of air like bioaerosol sampling, culture media for bacteria and fungi characterisation and for water analysis like MPN test, membrane filter technique. 4. Learn the different control measures against bioaerosols and water borne pathogens.

COURSE TITLE: AIR AND WATER MICROBIOLOGY

TOTAL HOURS: 45 CREDITS: 3

Unit 1. Aeromicrobiology No of hours:10

Bioaerosols, Air borne microorganisms (bacteria, Viruses, fungi) and their impact on human health and environment, significance in food and pharma industries and operation theatres, allergens

Unit 2. Sample Collection and Analysis No of Hours: 10

Bioaerosol sampling, air samplers, methods of analysis, CFU, culture media for bacteria and fungi, Identification characteristics.

Unit 3. Water Microbiology No of Hours: 10

Water borne pathogens, water borne diseases (Cholera, Typhoid and Amoebiasis)

Unit 4. Microbiological Analysis of Water No of Hours: 10

Sample Collection, Treatment and safety of drinking (potable) water, methods to detect potability of water samples: (a) standard qualitative procedure: presumptive/MPN tests confirmed and completed tests for faecal coliforms (b) Membrane filter technique and (c) Presence/absence tests

Unit 5. Control Measures No of Hours: 5

Fate of bioaerosols, inactivation mechanisms – UV light, HEPA filters, desiccation, Incineration; Water- Precipitation, chemical disinfection, filtration, high temperature.

SUGGESTED READINGS

1. Da Silva N, Taniwaki MH, Junqueira VC, Silveira N, Nascimento MS, Gomes RAR (2012) Microbiological Examination Methods of Food and WaterA Laboratory Manual, CRC Press
2. Atlas RM and Bartha R. (2000). Microbial Ecology: Fundamentals & Applications. 4th edition. Benjamin/Cummings Science Publishing, USA.
3. Maier RM, Pepper IL and Gerba CP. (2009). Environmental Microbiology. 2nd edition, Academic Press
4. Hurst CJ, Crawford RL, Garland JL, Lipson DA (2007) Manual of Environmental Microbiology, 3rd edition, ASM press.